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Amino acid analysis

For athletes or people with amino acid metabolism disorders, products with specific amino acid composition are a must. This requires a correct product declaration and consumer information.

In our laboratories we determine the exact composition of an amino acid profile (including taurine). Our spectrum includes, for example, dietary supplements, sports nutrition, foods for special medical purposes (FSMP), baby food, animal products (e.g. meat, fish, eggs, milk and dairy products), soy, cereals and cereal products, protein isolates, juices, energy drinks or the amino acids themselves.

In addition to the analysis of free amino acids, the analysis of bound amino acids (especially proteins) poses high analytical requirements in order to make the individual amino acids accessible for analysis. Our laboratories offer this valid amino acid analysis accredited.

Analysis of amino acids in food

The following methods are offered in our ISO 17025 accredited laboratories:

  • In-house method using microwave technology and HPLC-MS/MS for time and cost-efficient determination. Compounds such as taurine, hydroxyproline, hydroxylysine, gamma-aminobutyric acid (GABA) or ornithine are also analyzed, some of which cannot be detected by classical methods.
  • Analysis according to classical methods such as § 64 LFGB (ASU) L 49.07 or VO(EG) 152/2009 (identical to DIN EN ISO 13903 and 13904)

We are at your disposal for the selection of the right analytics and the interpretation of the results.

Free amino acid analysis

Depending on the method: alanine, arginine, asparagine, aspartic acid, cystine, gamma aminobutyric acid (GABA), glutamine, glutamic acid, glycine, histidine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, ornithine, phenylalanine, proline, serine, taurine, threonine, tryptophan, tyrosine, valine

Analysis of bound amino acids

Depending on the method: alanine, arginine, aspartic acid, cyst(e)in (sum of cystine + cysteine), gamma aminobutyric acid (GABA), glutamic acid, glycine, histidine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, ornithine, phenylalanine, proline, serine, taurine, threonine, tryptophan, tyrosine, valine

The following analytical methods are used (BG = limit of quantification):

  • SGS method (SOP M 3123):
    • Free amino acids: extraction of samples and analysis by HPLC-MS/MS (BG = 0.01 g/100g)
    • Bound amino acids (excluding tryptophan): Oxidation of the sample for determination of cyst(e)in and methionine, microwave digestion with hydrochloric acid and analysis by HPLC-MS/MS. (BG = 0.02 g/100g).
    • Bound tryptophan: microwave digestion with lithium hydroxide and analysis by HPLC-MS/MS (BG = 0.02 g/100g)
  • § 64 LFGB (ASU) L 49.07, VO (EG) 152/2009:
    • Free amino acids: extraction of samples and analysis by amino acid analyzer (ASA). (BG = 0.01 - 0.2 g/100g)
    • Bound amino acids (without tryptophan): Oxidation of sample for determination of cyst(e)in and methionine, digestion with hydrochloric acid and analysis by ASA. (BG = 0.01 - 0.3 g/100g)
    • Bound tryptophan: digestion with barium hydroxide and analysis by ASA (BG = 0.05 g/100g)

 

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SGS INSTITUT FRESENIUS GmbH

Tegeler Weg 33
10589 Berlin
t +49 30 346 07 700

Weidenbaumsweg 137
21035 Hamburg-Bergedorf

Im Maisel 14
65232 Taunusstein

Engesserstraße 4B
79108 Freiburg im Breisgau

SGS Analytics Germany GmbH

Orlaweg 2
07743 Jena
+49 3641 30963-0

Höhenstraße 24
70736 Fellbach
+49 711 16272 310